Document Type
Thesis - University Access Only
Award Date
1999
Degree Name
Master of Science (MS)
Department / School
Biology and Microbiology
Abstract
Wheat does not have a gene that can resist different aphid species on itself. I has been reported that potato proteinase inhibitor II is potentially effective protein for the control of these species. One major purpose of this research was to add a modified potato proteinase inhibitor II gene PI-IIK into wheat. Biolistics was employed to shoo he PI-IIK gene into wheat anther calli. The plants regenerated from these bombarded calli were screened for the transgene through PCR. 15 potentially transgenic fertile parts have been identified and further confirmed by semi-nested PCR. and Southen1 hybridization The transgene integration mechanisms were also studied. The results indicated that multiple copies of the transgene were integrated into the genomic DNA. The integration patterns include full length transgene(s) interspersed transgene(s), and rearranged transgenes Agrobacterium proteins might produce T-DNA-type insertion events in heat transformation through biolistics.
Library of Congress Subject Headings
Aphididae Wheat -- Genetics Wheat -- Disease and pest resistance
Format
application/pdf
Number of Pages
75
Publisher
South Dakota State University
Recommended Citation
Li, Zhuying, "Aphid Resistant Gene Transformation of Wheat by Biolistics" (1999). Electronic Theses and Dissertations. 642.
https://openprairie.sdstate.edu/etd2/642