Thesis - Open Access
Master of Science (MS)
Department / School
Agronomy, Horticulture, and Plant Science
E. Brent Turnipseed
Chromatography, HPLC, Rice, Seed Technology
High-performance liquid chromatography (HPLC) is a form of analytical chemistry where adsorption is a key factor in which the purpose is to separate, identify and quantify each component in a mixture. It is the optimal separation technique of chemical and biological compounds that are non-volatile including proteins and natural products (i.e., plant extracts). As each sample component interacts differently with the absorbent material within the column, the flow rates change leading to the separation of peaks, which are plotted via computer software. Most commonly HPLC analysis has been used for chemical manufacturing and pharmaceutical purposes. When using HPLC for crop variety identification or verification, the goal is not to look at identifying the individual protein components in each seed or to match peak height and width, as within the species the components should all be very similar. Instead, the analyst will match the peak pattern of the known variety standard in the chromatograph and the peak pattern of the unknown submitted sample, as each sample results in a unique pattern. Quantifying the peaks becomes challenging due to cultural practices (fertilization, irrigation, etc.), environmental factors (rainfall, temperature, etc.) and growth conditions (weed, pest, and disease pressure) that influence protein development and content within the seed. Due to this, the proteins among tested samples are variable, which is reflected in the chromatograms.
South Dakota State University
Jorgensen, Johnna, "Expanding the Use of High-Performance Liquid Chromatography for Varietal Identification or Verification of Selected Cereal Crops" (2023). Electronic Theses and Dissertations. 715.