Puja Agrawal

Document Type

Thesis - University Access Only

Award Date


Degree Name

Master of Science (MS)

Department / School



Resveratrol is a phytoalexin found in grapes. Epidemiological and experimental studies suggest that the consumption of wine, particularly red wine reduces the incidence and morbidity from coronary heart diseases. The purpose of this investigation is to study the in vitro antiperoxidant effect of resveratrol and compare with vitamin E, a known antioxidant. People using cardiovascular drugs may also consume resveratrol containing products for reducing the risk of disease. Thus, a potential interaction of resveratrol with the drugs is possible. Therefore, effects of resveratrol on the blood level of nifedipine, niacin, lovastatin in rats. IC50 values for resveratrol and vitamin E were l.5x10-5M and lxl0-3 M (NADPH induced) and l.5x104 M, lxl0-3 M (Ascorbate-induced) respectively. These results indicate that resveratrol has higher antiperoxidant effect than vitamin E. For the drug interaction experiments, nifedipine (antihypertensive), niacin (antilipemic) or lovastatin (antilipemic) were co-administered with resveratrol and serum prepared from blood obtained after one hour or one week was assayed using high performance liquid chromatography. Resveratrol pretreatment caused a 65% decrease in the concentration of nifedipine in both one hour and one week experiments. A modest increase (approximately 20%) in the level of niacin was observed in the presence of resveratrol at one hour and one week experiment. A decrease of 35% and 14.6% in the level of lovastatin was observed in both one hour experiment and one week experiment respectively. Ln order to elucidate the possible mechanism of action of these drug interaction, effects of resveratrol on rat liver microsomal drug metabolizing enzymes was investigated. Resveratrol caused a 22% decrease in cytochrome P450 3A in enzyme activity. This decrease may explain increased niacin levels but not the nifedipine and lovastatin. Nifedipine and lovastatin may be metabolized by different cytochrome P450 enzyme systems.

Library of Congress Subject Headings

Antioxidants Phytoalexins -- Physiological effect Wine -- Therapeutic use Drug interactions



Number of Pages



South Dakota State University