Document Type
Article
Publication Date
2-1-2009
Abstract
OBJECTIVE: To investigate whether adiposity influences endothelial progenitor cell (EPC) number and colony-forming capacity.
DESIGN: Cross-sectional study of normal weight, overweight and obese adult humans.
PARTICIPANTS: Sixty-seven sedentary adults (aged 45-65 years): 25 normal weight (body mass index (BMI) or=30 kg/m(2); 18 males/6 females). All participants were non-smokers and free of overt cardiometabolic disease.
MEASUREMENTS: Peripheral blood samples were collected and circulating EPC number was assessed by flow cytometry. Putative EPCs were defined as CD45(-)/CD34(+)/VEGFR-2(+)/CD133(+) or CD45(-)/CD34(+) cells. EPC colony-forming capacity was measured in vitro using a colony-forming unit (CFU) assay.
RESULTS: Number of circulating putative EPCs (either CD45(-)/CD34(+)/VEGFR-2(+)/CD133(+) or CD45(-)/CD34(+) cells) was lower (P<0.05) in obese (0.0007±0.0001%; 0.050±0.006%) compared with overweight (0.0016±0.0004%; 0.089±0.019%) and normal weight (0.0015±0.0003%; 0.082±0.008%) adults. There were no differences in EPC number between the overweight and normal weight groups. EPC colony-formation was significantly less in the obese (6±1) and overweight (4±1) compared with normal weight (9±2) adults.
CONCLUSION: These results indicate that: (1) the number of circulating EPCs is lower in obese compared with overweight and normal weight adults; and (2) EPC colony-forming capacity is blunted in overweight and obese adults compared with normal weight adults. Impairments in EPC number and function may contribute to adiposity-related cardiovascular risk.
Publication Title
International Journal of Obesity
Volume
33
Issue
2
First Page
219
Last Page
225
Pages
14
Type
text
Format
application/pdf
Language
en
PMCID
PMC2643316
DOI of Published Version
:10.1038/ijo.2008.262
Publisher
Nature Publishing
Rights
In Copyright - Non-Commercial Use Permitted
http://rightsstatements.org/vocab/InC-NC/1.0/
Recommended Citation
MacEneaney, O J; Kushner, E J; Van Guilder, Gary P.; Greiner, J J; Stauffer, B L; and DeSouza, C A, "Endothelial Progenitor Cell Number and Colony-forming Capacity in Overweight and Obese Adults" (2009). Health and Nutritional Sciences Faculty Publications. 61.
https://openprairie.sdstate.edu/hns_pubs/61
Comments
This is the HHS Public Access Authors Manuscript. The Version of Record was published in Int J Obes (Lond). 2009 February ; 33(2): 219–225. doi:10.1038/ijo.2008.262.