Faculty Mentor

S.A. Clay


About 479,000 pounds of 2,4-D (2, 4-dichlorophenoxyacetic acid) was applied to South Dakota's crops in 2000. 2,4-D can injure or reduce yield of soybean (Glycine max) if deposited on growing plants. This study determined uptake, translocation, and metabolism of ring-labeled-2,4-D in soybean at the third trifoliate (V3) stage of growth. Plants were harvested and partitioned into four parts from 1 hr (HAT)to 10 d (DAT) after treatment. Thin layer chromatography techniques were used to determine if remaining in the tissue was parent chemical or metabolite. 2,4-D uptake ranged from 39% at 1 HAT to 74% 6 DAT.By 10 DAT, translocated to the youngest tissue (10%) and older tissue (8%). All '''C recovered from the treated leaf 1 HATwas parent 2,4-D, however, at 24 HAT and later, only about30%of the '"C remained as 2,4-D whereas 70% of the '"C was observed as a more water soluble compound.



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