Document Type

Data set

Publication Date

6-7-2019

Dates of Collection

Original specimen collection occurred from 1936 to 1970> DNA sampling and extraction was performed in 2013

Description

Specimens from natural history collections (NHCs) are increasingly being used for genetic studies and can produce baseline data for populations of declining species. Differentiating among sample source quality can direct destructive sampling to the source predicted to yield the highest quality DNA and most reliable data, reducing damage to specimens, laboratory costs, and genotyping errors. We used the kit fox (Vulpes macrotis) as a model species and evaluated the quality and reliability of genetic data obtained from carnivoran specimens via three different sample sources: cranial bones, nasal bones, and toepads. We quantified variation in amplification success and genotyping error rates, and assessed the reliability of resulting data.

Funder(s)

T&E Incorporated’s Grants for Conservation Biology (No Grant Number)
U. S. Army Research Laboratory and the U. S. Army Research Office (RC-201205)
U.S. Department of Defense Environmental Security Technology Certification (12 EB-RC5-006)
Legacy Resource Management (W9132T-12-2-0050)

File Format(s)

Excel (.xlsx)

File Size

325 KB

Rights

© 2019 The Authors

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