Document Type
Article
Publication Version
Version of Record
Publication Date
12-2016
Keywords
Caryophyllaceae, microsatellites, plant–animal interaction, polyploidy, Silene stellata
Abstract
Premise of the study:We designed and tested microsatellite markers for the North American native species Silene stellata (Caryophyllaceae) to investigate its population genetic structure and identify selection on floral design through male reproductive success.
Methods and Results: A total of 153 candidate microsatellite loci were isolated based on next-generation sequencing. We identified 18 polymorphic microsatellite loci in three populations of S. stellata, with di- or trinucleotide repeats. Genotyping results showed the number of alleles per locus ranged from six to 45 and expected heterozygosity ranged from 0.511 to 0.951. Five of these loci were successfully amplified in S. virginica and S. caroliniana and were also polymorphic.
Conclusions: The microsatellite markers reported here provide a valuable tool for paternity analysis in S. stellata. They will also be useful for investigating the population genetic structures of S. stellata and related species.
Publication Title
Applications in Plant Science
Volume
4
Issue
12
Pages
5
Format
application/pdf
Language
en
DOI of Published Version
10.3732/apps.1600117
Publisher
Botanical Society of America
Rights
This article is a U.S. Government work and is in the public domain in the USA.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Share Alike 3.0 License.
Recommended Citation
Zhou, Juannan; Dudash, Michele R.; Fenster, Charles B.; and Zimmer, Elizabeth A., "Development of Highly Variable Microsatellite Markers for the Tetraploid Silene stellata (Caryophyllaceae)" (2016). Natural Resource Management Faculty Publications. 222.
https://openprairie.sdstate.edu/nrm_pubs/222