Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map

Authors

Dilmini Alahakoon, South Dakota State UniversityFollow
Anne Fennell, South Dakota State UniversityFollow
Zachary Helget, South Dakota State UniversityFollow
Terry Bates, Cornell University
Avainash Karn, Cornell University
David Manns, Cornell University
Anna Katherine Mansfield, Cornell University
Bruce I. Reisch, Cornell University
Gavin Sacks, Cornell University
Qi Sun, Cornell University
Cheng Zou, Cornell University
Lance Cadle-Dvidson, USDA-ARS
Jason P. Londo, Cornell University

Document Type

Article

Publication Date

2022

Abstract

Increased map density and transferability of markers are essential for the genetic analysis of fruit quality and stress tolerance in interspecific grapevine populations. We used 1449 GBS and 2000 rhAmpSeq markers to develop a dense map for an interspecific F₂ population (VRS-F₂) that was derived by selfing a single F₁ from a Vitis riparia x ‘Seyval blanc’ cross. The resultant map contained 2519 markers spanning 1131.3 cM and was highly collinear with the Vitis vinifera ‘PN40024’ genome. Quantitative trait loci (QTL) for berry skin color and flower type were used to validate the map. Four rhAmpSeq transferable markers were identified that can be used in pairs (one pistillate and one hermaphroditic) to predict pistillate and hermaphrodite flower type with ≥99.7% accuracy. Total and individual anthocyanin diglucoside QTL mapped to chromosome 9 near a 5-O-GLUCOSYLTRANSFERASE candidate gene. Malic acid QTL were observed on chromosome 1 and 6 with two MALATE DEHYRDROGENASE CYTOPLASMIC 1 and ALUMINUM-ACTIVATED MALATE TRANSPORTER 2-LIKE (ALMT) candidate genes, respectively. Modeling malic acid identified a potential QTL on chromosome 8 with peak position in proximity of another ALMT. A first-ever reported QTL for the grassy smelling volatile (E)-2-hexenal was found on chromosome 2 with a PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE candidate gene near peak markers.

Publication Title

Plants

Volume

11

Issue

5

DOI of Published Version

10.3390/plants11050696

Publisher

MDPI

Rights

Copyright © the Authors

Recommended Citation

Alahakoon D, Fennell A, Helget Z, Bates T, Karn A, Manns D, Mansfield AK, Reisch BI, Sacks G, Sun Q, Zou C, Cadle-Davidson L, Londo JP. Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map. Plants. 2022; 11(5):696. https://doi.org/10.3390/plants11050696

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.