Toward Genetic Engineering: Teaching Transformation Using the pGLO Plasmid in High School Classrooms
Document Type
Article
Publication Version
Published
Publication Date
Fall 9-10-2022
Keywords
Transformation, Genetic Engineering, Biotechnology, Microbiology, Agar Art
Description
In this lesson plan, students will learn about genetic engineering and perform the same transformation protocol used by scientists on a daily basis around the world. Genetic engineering is a technique used for direct manipulation, alteration, or modification of genes or genomes of an organism to manipulate the phenotypes. The use of genetically modified organisms (GMOs) has emerged everywhere as a mainstay from agriculture to pharmaceuticals. Through this lesson, students will transform Escherichia coli with pGLO plasmid, which give a brilliant fluorescent green glow under UV light. Students will also learn the central dogma of molecular biology: DNA à RNA à Protein à Trait (Green Fluorescence), and the basics of gene regulation. This hands-on module is designed for 9th through 12th grade students. To adjust the difficulty level of the modules, some aspects can be modified or removed based on the grade. This module includes an instructional slide show, assessment options, and links to additional resources.
Pages
21
Type
Teaching Resource
Format
PDF & PPT
Language
English
Publisher
South Dakota State University
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Recommended Citation
Isaac Kovash, Tahmina Hossain, Michael Mitchell, Sudeshna Roy, Madhav P. Nepal, and Nicholas C. Butzin. 2022. Exploring Bacterial Diversity through Hand-printing & Bacterial Art. ILEARN Teaching Resources. 4 (2): 106—125