Document Type
Article
Publication Date
3-15-2015
Keywords
porcine, epidemic, diarrhea, virus, PEDV, inactivated, vaccine, immunogenicity
Abstract
Background: Porcine epidemic diarrhea virus (PEDV), a highly pathogenic and transmissible virus in swine, was first detected in the U.S. in May, 2013, and has caused tremendous losses to the swine industry. Due to the difficulty in isolating and growing this virus in cell culture, few vaccine studies using cell culture propagated PEDV have been performed on U.S. strains in pigs. Therefore, the objective of this study was to evaluate the humoral immune response to the selected inactivated PEDV vaccine candidate in a dose-titration manner.
Results: PEDV was isolated from a pig with diarrhea and complete genome sequencing found >99% nucleotide identity to other U.S. PEDV. Inactivated adjuvanted monovalent vaccines were administered intramuscularly to five week old pigs in a dose titration experimental design, ranging from 6.0-8.0 log10 tissue culture infective dose (TCID50/mL), to evaluate immunogenicity using a fluorescent foci neutralization assay (FFN), fluorescent microsphere immunoassay (FMIA), and enzyme-linked immunosorbent assay (ELISA) on sera. Pigs vaccinated with 8.0 log10 TCID50/mL inactivated virus showed significantly higher FFN titers as well as FMIA and ELISA values than 6.0 log10 TCID50/mL vaccinates and the negative controls.
Conclusions: These results demonstrate the immunogenicity of a PEDV inactivated viral vaccine with a U.S. strain via dose-titration. A future vaccination-challenge study would illustrate the efficacy of an inactivated vaccine and help evaluate protective FFN titers and ELISA and FMIA responses.
Publication Title
BMC Veterinary Research
Volume
11
Issue
62
Pages
9
Language
en
DOI of Published Version
10.1186/s12917-015-0357-1
Publisher
BioMed Central
Rights
Copyright © Collin et al.
Recommended Citation
Collin, Emily A.; Anbalagan, Srivishnupriya; Okda, Faten; Bateman, Ron; Nelson, Eric A.; and Hause, Ben M., "An Inactivated Vaccine Made From a U.S. Field Isolate of Porcine Epidemic Disease Virus is Immunogenic in Pigs as Demonstrated by a Dose-titration" (2015). Veterinary and Biomedical Sciences Faculty Publications. 2.
https://openprairie.sdstate.edu/vbs_pubs/2
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Comments
This article was published in BMC Veterinary Research. 11:62.
Posted with permission.