2,4-Thiazolidinedione in Well-Fed Lactating Dairy Goats: I. Effect on Adiposity and Milk Fat Synthesis

Document Type

Article

Publication Date

2019

Journal

Vet Sci.

Volume

6

Issue

2

Pages

pii: E45

Language

en.

Keywords

2, 4-thiazolidinedione; goat; milk fat; metabolism

Abstract

Background: In a prior experiment, treatment of goats with the putative PPARγ agonist 2,4-thiazolidinedione(2,4-TZD)didnotaffectmilkfatorexpressionofmilk-fatrelatedgenes. Thelack of response was possibly due to deficiency of vitamin A and/or a poor body condition of the animals. In the present experiment, we tested the hypothesis that PPARγ activation affects milk fat synthesis in goats with a good body condition and receiving adequate levels of vitamin A. Methods: Lactating goats receiving a diet that met NRC requirements, including vitamin A, were injected with 8 mg/kg BW of 2,4-TZD (n = 6) or saline (n = 6; CTR) daily for 26 days. Blood metabolic profiling and milk yield and components were measured including fatty acid profile. Expression of genes related to glucose and lipid metabolism was measured in adipose tissue and in mammary epithelial cells (MEC). Size of adipocytes was assessed by histological analysis. Results: NEFA, BHBA, and fatty acids available in plasma decreased while glucose increased in 2,4-TZD vs. CTR. Size of cells and expressionofinsulinsignalingandglucosemetabolism-relatedgeneswerelargerin2,4-TZDvs. CTR in adipose tissue. In MEC, expression of SCD1 and desaturation of stearate was lower in 2,4-TZD vs. CTR. Conclusions: Overall data revealed a lack of PPARγ activation by 2,4-TZD and no effect on milk fat synthesis despite a strong anti-lipolysis effect on adipose tissue.

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