Improving the extraction of dairy phospholipids by the combined effect of ultrasound pretreatment and switchable solvents

Document Type

Abstract

Publication Date

2020

Publisher

American Dairy Science Association

Journal

Journal of Dairy Science

Volume

103

Issue

Suppl. 1

Pages

6

Language

en

Keywords

switchable solvent, ultrasound, phospholipids

Abstract

In the last decade, the consumption of phospholipids (PLs), a class of polar lipids, has been associated with important health benefits. Dairy by-products are an abundant source of PLs with potential for extraction and isolation. In this work, we report improvements in the extraction efficiency of PLs from Beta-serum, a dairy by-product, by the application of ultrasound (USD) before the extraction with switchable hydrophilicity solvent (SHS), these solvents can switch from hydrophobic to hydrophilic form by inserting the CO2 .The ultrasound pretreatment consisted of the application of 3 levels of acoustic intensity (either 134.15 ± 1.20, 274.91 ± 2.47, or 385.67 ± 3.47 W/m−2) for 4 min. After the pretreatment, the extraction of the lipid fraction was performed using a tertiary amine (N,N-dimethylcyclohexylamine, CyNMe2) as a switchable hydrophilicity solvent (SHS) at 3 different sample to SHS ratios (1/3, 1/6 and 1/12). The PLs were recovered by solid phase-microextraction, and the individual PLs were quantified by HPLC with charged aerosol detector. The effect of acoustic intensity (385.67 ± 3.47 W/m−2) followed by SHS extraction using 1/12 ratio recovered 69.07 ± 0.11% of PLs, while only 7.57 ± 0.59% of the PLs were recovered without the ultrasound pretreatment. The recovered fraction of PLs mainly comprised of phosphatidylinositol (32%), phosphatidylethanolamine (30%), and sphingomyelin (37%) found higher compared with untreated samples. Scanning electron images, particle size, and gel electrophoresis revealed great disruption of the protein matrix that may release the PLs into the aqueous medium. The application of ultrasound prior the SHS extraction remarkably improved the recovery efficiency of PLs. The proposed method improves the extraction of dairy PLs and may help to expand the utilization of thereof.

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