Document Type
Thesis - University Access Only
Award Date
2006
Degree Name
Master of Science (MS)
Department / School
Agronomy
Abstract
The use of host plant resistance is a major component for managing stem rust caused by Puccinia graminis f. sp. tritici, in wheat (Triticum aestivum L.). In this study, a segregating population from a cross between 2137 and CR L-Sr35 was made to develop a molecular marker for the stem rust resistant gene Sr 35. Ninety-eight F2 plants were inoculated with a stem rust isolate of race QTH. Infection type and severity were evaluated 10 days after inoculation. Twenty-one microsatellite primer pairs developed previously from the long arm of chromosome 3A were used to determine polymorphism among parental lines and the F2 population. Chi square analysis indicated that resistance to stem rust was conferred by a dominant gene (J:=l.01, P=0.48). Only four primers (Xgwm155, Xgwm391, Xgwrn497 and Xfca2076) revealed polymorphism and they were used for linkage analysis. Primer pair Xgwm391 revealed a polymorphic fragment of 200 bp found only in the susceptible bulk and parent 2137. In contrast, primer pair Xcfa2076 amplified polymorphic fragments of 210 bp in the resistant bulk and CR L-Sr35 and 200 hp in the susceptible bulk and 2137. Chi square analysis revealed that the Xcfa2076 marker was associated with the Sr 35 resistance gene and no significant deviation from the expected ratio was observed (i=0.78; P=0.4). The microsatellite marker Xfca2076 can be exploited for selecting Sr35 in wheat cultivars using marker assisted selection and combining this gene with other resistance genes, such as Sr 2, Sr24 and Sr 31, which are considered among the primary sources of resistance in most of the Northern Plains wheat varieties.
Library of Congress Subject Headings
Wheat -- Disease and pest resistance -- Genetic aspects
Puccinia graminis
Wheat rusts
Microsatellites (Genetics)
Genetic markers
Format
application/pdf
Number of Pages
50
Publisher
South Dakota State University
Recommended Citation
Babiker, Ebrahiem M., "Development of a Microsatellite Marker Associated with Stem Rust Resistance Gene Sr35 in Wheat" (2006). Electronic Theses and Dissertations. 1241.
https://openprairie.sdstate.edu/etd2/1241