Document Type

Thesis - University Access Only

Award Date

1995

Degree Name

Master of Science (MS)

Department / School

Chemistry

First Advisor

Padmanaban G. Krishnan

Abstract

Conventional analytical procedures for determination of niacin or vitamin B3 in foods are tedious, use high levels of toxic chemicals, and are time consuming. In addition, food matrices are difficult samples owing to their complex nature. Also the vitamins occur at low levels. A selective and sensitive HPLC technique was developed using postcolumn derivatization as well as fluorescence and visible detection systems. Niacin was separated and retained for 6 minutes on a polymeric column employing an aqueous mobile phase containing sodium acetate buffer. The postcolumn system consisting of a stainless steel pump and a reaction coil allowed detection and quantitation of niacin. Ready to eat cereals were analyzed using HPLC and official AACC methods. The lower detection limit and mean recovery were 3.6 ng and 99.43% respectively. The coefficient of determination (R-square) was 96.12%. There was no significant difference observed between the official AACC method and HPLC fluorimetric and visible absorption methods.

Library of Congress Subject Headings

Niacin
Cereals, Prepare -- Analysis
High performance liquid chromatography
Food -- Vitamin content

Format

application/pdf

Publisher

South Dakota State University

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Rights Statement

In Copyright