Document Type
Thesis - Open Access
Award Date
2008
Degree Name
Master of Science (MS)
Department / School
Biology and Microbiology
Abstract
Japanese wheat cultivar "Tokai-66" was profiled for resistance Quantitative Trait Loci (QTL) pertaining to Fusarium Head Blight (FHB), a devastating disease of wheat and barley resulting in reduction of yield, and contamination of grain with mycotoxins. This was accomplished with a population (175 F2 7) of Recombinant Inbred Lines derived from a cross between Tokai-66 and a susceptible Tibetan land race known as Yl 193-6. The disease response was recorded over three greenhouse trials. FHB was initiated with single floret injections of IO μL of a I 00,000 conidia per milliliter of inoculum. After inoculation, heads were incubated in moist Ziploc bags for 72 hours. Heads were observed for spread of disease after 21 days. Kernels were observed for the percent that were damaged by Fusarium, and then sent to the University of Minnesota for Deoxynivalcnol (DON, a mycotoxin) content measurements. Known DNA polymorphisms between the parents were tested using bulk analysis on lines exhibiting consistent resistance or susceptibility. Individual analysis was then performed with four markers on 3BS, as 3BS was found to have high correlation to resistance. The I Band 7DL seem to be resistance sources from the susceptible parent, Y 1193-6. These data were subjected to normality analysis, and interval mapping tests using Excel, JMP, SAS, and MapManagerQTX. Despite the tests returning non-normal distributions, Tokai-66 seems to have novel resistance to FHB. More study should be performed in order to further characterize the resistance sources on the aforementioned arms, to alleviate statistical insignificance, and to validate the map distances found between the 3BS markers used in this study.
Library of Congress Subject Headings
Wheat -- Disease and pest resistance -- Genetic aspects
Wheat -- Genome mapping
Fusarium diseases of plants
Fungal diseases of plants
Format
application/pdf
Number of Pages
95
Publisher
South Dakota State University
Recommended Citation
Wiedel, Noah A., "Identification of FHB Resistance QTL in Tokai-66" (2008). Electronic Theses and Dissertations. 1486.
https://openprairie.sdstate.edu/etd2/1486
