"Development of Food Preservative Hydrogel Using Bioactive Compounds Ex" by Kayla Christopherson

Document Type

Thesis - Open Access

Award Date

2025

Degree Name

Master of Science (MS)

Department / School

Agricultural and Biosystems Engineering

First Advisor

Zhengrong Gu

Abstract

With an ever-growing population comes the need for a greater food supply. Yet land and other agricultural resources put a limit on the availability of food production. As farmers strive to optimize higher produce yields, society must turn to greener, more sustainable food preservation techniques to reduce food spoilage. The inclusion of extracted bioactive compounds found in canola meal into a food preservative hydrogel is such a solution for food spoilage reduction. In this thesis, extraction parameters of glucosinolates were examined along with their inhibition of E. coli DH5αZ1 when incorporated into 2 developed hydrogels: gelatin A Schiff-base and agar-agar. The optimal extraction parameters used a1000 μm particle size for 15 min. at 50°C with a 1:5 solid-to-liquid loading ratio and pH 3.0 buffer solution as liquid volume for a total glucosinolate amount of 925.68 μmol. The gelatin A Schiff-base hydrogel showed possible inhibition but was ineffective for industrial use, whereas the agar-agar hydrogel did not have visible bacterial inhibition.

Publisher

South Dakota State University

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Rights Statement

In Copyright