Document Type
Thesis - University Access Only
Award Date
1996
Degree Name
Master of Science (MS)
Department / School
Biology and Microbiology
Abstract
Cationic lipopolyamines have been successfully used for transformation of mammalian tissue. Therefore, wheat and barley callus and suspension cultures were electroporated to determine if cationic lipopolyamines would increase the efficiency of gene delivery into cereals. Experiments were performed with plasmid pAHC27 which has the gus gene under the control of the ubiquitin promoter. Spectrophotometric and 2histochemical GUS assays revealed higher levels of GUS activity in the tissues electroporated with DNA in the presence of cationic lipopolyamines. Two lipopolysomal reagents, Transfectam and Lipofectamine, were compared. Transfectam caused an increase in GUS activity as large as 48 times that of DNA only when the assay was performed after 6 hours. The efficiency of transformation in the presence of Lipofectamine was 4 times greater for wheat (SD 16029) than for barley (Dicktoo). This is the first description of cationic lipopolyamine electroporation mediated transfer of DNA into cereals. We believe that this protocol will be a successful alternative to other transformation techniques.
Library of Congress Subject Headings
Grain -- Genetics
Electroporation
DNA
Liposomes
Format
application/pdf
Number of Pages
67
Publisher
South Dakota State University
Recommended Citation
Isaac, Kalpana, "Synthetic Cationic Lipopoyamines Enhance Electroporation-Mediated DNA Transfer into Cereals" (1996). Electronic Theses and Dissertations. 257.
https://openprairie.sdstate.edu/etd2/257
Rights Statement
