Zaikai Li

Document Type

Thesis - University Access Only

Award Date

1997

Degree Name

Master of Science (MS)

Department / School

Animal Science

Abstract

Seventy nine myogenic satellite cell clones were established from the pectoralis major muscle (PM) of chickens with the Low Score Normal (LSN) muscle weakness and Controls, using a Quixell robotic cell transfer system. LSN birds are characterized as having difficulty in righting themselves from a supine position. The percentage of cells which attached to substrata and began to proliferate was higher for the Control line than the LSN line (53% vs. 29%). Furthermore, of those clones which initiated growth, 64 % of the Control cells and only 33% of the LSN cells proliferated to confluency in 25 cm2 flasks. Clones possessing the fastest growth rates, good cell morphology and ability to form myotubes were chosen from both lines for further study. Of the eight different combinations of media, sera and growth factors tested, McCoys 5A + 10% chicken serum (CS)+ 5% horse serum (HS)+ 20 ng/ml fibroblast growth factor (FGF) was shown to support the greatest rates of chicken satellite cell proliferation. Using this medium, proliferation rates were significantly lower with LSN satellite cells than with Controls (P<0.05). A chicken serum dose response curve conducted between 0 and 10% CS in the presence of 5% HS demonstrated that LSN satellite cells were less responsive to the mitogenic effects of chicken serum at all levels tested (P<0.05). Differentiation rates of LSN satellite cells (as measured by creatine kinase levels) in low serum containing medium were significantly lower compared with Controls (P<0.05). There was a greater (P<0.05) number of insulin-like growth factor receptors on LSN satellite cells compared to Controls. However, the IGF receptor binding affinities (Kds) were similar (P>0.05) between the two cell lines. Protein synthesis rates between Control and LSN satellite cells were similar, but protein degradation rates were higher in the LSN satellite cells. The results suggest that a defect in satellite cell physiology may contribute to the skeletal muscle weakness seen in the LSN line.

Library of Congress Subject Headings

Chickens -- Physiology

Satellite cells

Muscle cells

Muscle strength

Format

application/pdf

Number of Pages

62

Publisher

South Dakota State University

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