Document Type
Thesis - University Access Only
Award Date
1999
Degree Name
Master of Science (MS)
Department / School
Animal Science
Abstract
The effect of hepatocyte growth factor (HGF) on turkey satellite cell proliferation and differentiation was examined in cell culture. Satellite cell clones were established from a single muscle from an individual turkey. Results show that HGF is a potent activator and mitogen of turkey satellite cells and embryonic myoblasts with maximal stimulation at a level of 1 ng/ml. HGF is also an inhibitor of differentiation of turkey satellite cells. There is heterogeneity in responsiveness to HGF in the turkey satellite cell population. The "Late" clone, which reached confluence in a 25 cm2 flask after 29 days of total growth, was more responsive to the mitogenic effects of HGF than the "Early" clone (P < .05), which reached confluence on day 19. However, two other early clones exhibited similar response to the stimulating effects of HGF on proliferation as two other late clones. When combined with IGF and FGF, singularly or in combination, HGF did not exert additive or synergistic effects on either Early or Late clones. Whereas, when combined with IGF, FGF, and PDGF, HGF stimulated proliferation of the Late clone (P<0.05), but not the Early clone. Addition of anti-HGF antibody to culture media provided evidence of autocrine production of HGF by turkey satellite cell cultures. The results demonstrate that heterogeneity also exists in the turkey satellite cell population with respect to autocrine production of HGF.
Library of Congress Subject Headings
Hepatocyte growth factor Satellite cells Muscle cells Turkeys -- Growth
Format
application/pdf
Number of Pages
110
Publisher
South Dakota State University
Recommended Citation
Zeng, Caiyun, "The Effect of Hepatocyte Growth Factor (HGF) on Turkey Satellite Cell Proliferation and Differentiation" (1999). Electronic Theses and Dissertations. 662.
https://openprairie.sdstate.edu/etd2/662