Document Type

Thesis - University Access Only

Award Date

2002

Degree Name

Master of Science (MS)

Department / School

Biology and Microbiology

Abstract

Stevens, Elliot Cells supporting the replication of porcine reproductive and respiratory syndrome virus (PRRSV) die through a non-typical form of apoptosis that precedes necrosis. Cells transfected with GP5-enhanced green fluorescent protein (EGFP) were evaluated for viability and evidence of apoptosis from I to 7 days post transfection ( dpt). Ninety eight percent of cells expressing the full-length GP5 died between 1 to 7 dpt. We prepared various constructs lacking all or part of the ectodomain, transmembrane and/or endodomain of GP5. The results indicate that the transmembrane domain and the endodomain are responsible for cell death. In an attempt to characterize the mechanism of cell death, we used DNA fragmentation and chromatin condensation assays. These experiments failed to reveal features associated with apoptosis. Like the full-length virus, it could be possible that expression of the GP5 results in a unique form of cell death.

Library of Congress Subject Headings

Porcine reproductive and respiratory syndrome Swine -- Virus diseases Cell death

Format

application/pdf

Number of Pages

93

Publisher

South Dakota State University

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