Document Type
Article
Publication Version
Version of Record
Publication Date
2017
Abstract
Premise of the study: Chloroplast primers were developed from genomic data for the taxonomically challenging genus Castilleja. We further tested the broader utility of these primers across Orobanchaceae, identifying a core set of chloroplast primers amplifying across the clade.
Methods and Results: Using a combination of three low-coverage Castilleja genomes and sequence data from 12 Castilleja plastomes, 76 primer combinations were specifically designed and tested for Castilleja. The primers targeted the most variable portions of the plastome and were validated for their applicability across the clade. Of these, 38 primer combinations were subsequently evaluated in silico and then validated across other major clades in Orobanchaceae.
Conclusions: These results demonstrate the utility of these primers, not only across Castilleja, but for other clades in Orobanchaceae— particularly hemiparasitic lineages—and will contribute to future phylogenetic studies of this important clade of parasitic plants.
Publication Title
Applications in Plant Sciences
Volume
5
Issue
9
Format
application/pdf
DOI of Published Version
10.3732/apps.1700020
Publisher
Botanical Society of America
Rights
Copyright © the Authors.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Recommended Citation
Latvis, Maribeth; Mortimer, Sebastian M. E.; Morales-Briones, Diego F.; Torpey, Samuel; Uribe-Convers, Simon; Jacobs, Sarah J.; Mathews, Sarah; and Tank, David C., "Primers for Castilleja and their Utility Across Orobanchaceae: I. Chloroplast Primers" (2017). Natural Resource Management Faculty Publications. 294.
https://openprairie.sdstate.edu/nrm_pubs/294