Document Type
Article
Publication Version
Version of Record
Publication Date
2017
Abstract
Premise of the study: We developed primers targeting nuclear loci in Castilleja with the goal of reconstructing the evolutionary history of this challenging clade. These primers were tested across other major clades in Orobanchaceae to assess their broader utility.
Methods and Results: We assembled low-coverage genomes for three taxa in Castilleja and developed primer combinations for the single-copy conserved ortholog set (COSII) and the pentatricopeptide repeat (PPR) gene family. These primer combinations were designed to take advantage of the Fluidigm microfluidic PCR platform and are well suited for high-throughput sequencing applications. Eighty-seven primers were designed for Castilleja, and 27 were found to have broader utility in Orobanchaceae.
Conclusions: These results demonstrate the utility of these primers, not only across Castilleja, but for other lineages within Orobanchaceae as well. This expanded molecular toolkit will be an asset to future phylogenetic studies in Castilleja and throughout Orobanchaceae.
Publication Title
Applications in Plant Sciences
Volume
5
Issue
9
First Page
1700038
Format
application/pdf
DOI of Published Version
10.3732/apps.1700038
Publisher
Botanical Society of America
Rights
© 2017 the Authors
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 International License.
Recommended Citation
Latvis, Maribeth; Jacobs, Sarah J.; Mortimer, Sebastian M. E.; Richards, Melissa; Blischak, Paul D.; Mathews, Sarah; and Tank, David C., "Primers for Castilleja and their Utility Across Orobanchaceae: II. Single‐copy nuclear loci" (2017). Natural Resource Management Faculty Publications. 295.
https://openprairie.sdstate.edu/nrm_pubs/295