Transcriptomic Response is More Sensitive to Water Deficit in Shoots than Roots of Vitis riparia (Michx.)

Authors

Vedbar Singh Khada, South Dakota State University
Kimberly Vaughn, South Dakota State University
Juan Xie, South Dakota State UniversityFollow
Padmapriya Swaminathan, South Dakota State UniversityFollow
Qin Ma, South Dakota State UniversityFollow
Grant Cramer, University of Nevada, Reno
Anne Y. Fennell Dr, South Dakota State UniversityFollow

Document Type

Article

Publication Date

2-2019

Abstract

Background: Drought is an important constraint on grapevine sustainability. Vitis riparia, widely used in rootstock and scion breeding, has been studied in isolated leaf drying response studies; however, it is essential to identify key root and shoot water deficit signaling traits in intact plants. This information will aid improved scion and rootstock selection and management practices in grapevine. RNAseq data were generated from V. riparia roots and shoots under water deficit and well-watered conditions to determine root signaling and shoot responses to water deficit.
Results: Shoot elongation, photosynthetic rate, and stomatal conductance were significantly reduced in water deficit (WD) treated than in well-watered grapevines. RNAseq analysis indicated greater transcriptional differences in shoots than in roots under WD, with 6925 and 1395 genes differentially expressed, respectively (q-value < 0.05). There were 50 and 25 VitisNet pathways significantly enriched in WD relative to well-watered treatments in grapevine shoots and roots, respectively. The ABA biosynthesis genes beta-carotene hydroxylase, zeaxanthin epoxidase, and 9-cis-epoxycarotenoid dioxygenases were up-regulated in WD root and WD shoot. A positive enrichment of ABA biosynthesis genes and signaling pathways in WD grapevine roots indicated enhanced root signaling to the shoot. An increased frequency of differentially expressed reactive oxygen species scavenging (ROS) genes were found in the WD shoot. Analyses of hormone signaling genes indicated a strong ABA, auxin, and ethylene network and an ABA, cytokinin, and circadian rhythm network in both WD shoot and WD root.
Conclusions: This work supports previous findings in detached leaf studies suggesting ABA-responsive binding factor 2 (ABF2) is a central regulator in ABA signaling in the WD shoot. Likewise, ABF2 may have a key role in V. riparia WD shoot and WD root. A role for ABF3 was indicated only in WD root. WD shoot and WD root hormone expression analysis identified strong ABA, auxin, ethylene, cytokinin, and circadian rhythm signaling networks. These results present the first ABA, cytokinin, and circadian rhythm signaling network in roots under water deficit. These networks point to organ specific regulators that should be explored to further define the communication network from soil to shoot

Publication Title

BMC Plant Biology

Volume

19

Issue

72

DOI of Published Version

10.1186/s12870-019-1664-7

Publisher

Springer Nature Limited

Recommended Citation

Khadka, V.S., Vaughn, K., Xie, J. et al. Transcriptomic response is more sensitive to water deficit in shoots than roots of Vitis riparia (Michx.). BMC Plant Biol 19, 72 (2019) doi:10.1186/s12870-019-1664-7

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