Document Type
Dissertation - University Access Only
Award Date
1990
Degree Name
Doctor of Philosophy (PhD)
Department / School
Animal Science
Abstract
Quality of proteins after partial degradation and conversion into microbial protein was evaluated for lambs fed a corn cob based semipurified diet. Three animal experiments were conducted to measure nitrogen balance and plasma or blood free amino acids in lambs fed purified protein sources differing in rumen degradability. Before amino acid concentrations could be analyzed by gas chromatography as heptafluorobutyryl, isobutyl esters, methods for extracting free amino acids from plasma were evaluated. Either plasma dilution with 1 N acetic acid (1: 1 O, vol:vol) or deproteinization using 10% sulfosalicyclic acid (1: 1, vol:vol) prior to solid phase extraction was determined adequate. Experiment 1. Casein, soy protein isolates and zein included at 12% and urea included at 4.3% in the semipurified diet were fed at 2.2% body weight to lambs. Apparent biological value was greater (P<.05) lysine. An amino acid ratio was used to determine limiting amino acids. For urea diets, leucine and lysine were identified as first and second limiting, respectively. For zein diets, lysine was identified as first limiting.
Library of Congress Subject Headings
Ruminants -- Feeding and feeds Lambs -- Feeding and feeds Amino acids in animal nutrition Proteins in animal nutrition
Format
application/pdf
Number of Pages
84
Publisher
South Dakota State University
Recommended Citation
Hoppe, Karl F., "Effect of Protein Source on Amino Acid Nutrition in Ruminants Using a Semipurified Diet" (1990). Electronic Theses and Dissertations. 1171.
https://openprairie.sdstate.edu/etd2/1171