Document Type
Thesis - University Access Only
Award Date
2009
Degree Name
Master of Science (MS)
Department / School
Biology and Microbiology
Abstract
Phytochromes are essential controllers in various light responsive plant processes including dormancy induction and cold hardiness responses. While phytochrome A (PHY A) and B (PHYB) are well studied, less is known about other family members. This study characterized phytochrome E (PHYE) in the photoperiod responsive and cold hardy species Vitis riparia. PHYE, closely related phylogenetically to PHYB and phytochrome D, has been shown to play a role in the shade avoidance syndrome, resulting in increased apical dominance, stem elongation and altered flowering time. The naturally occurring levels of PHYE and their role in development have not yet been examined in grapevines. Thus, the PHYE genomic sequence was aquired and compared with other phytochromes to determine their relationships. PHYE expression was measured by Real-Time PCR in shoot tip, leaf, node, intemode, tendril and root at various tissue maturity levels to study the possible effect PHYE has on the vine. Leaf data was variable and greater sample numbers will be needed for conclusive results. Node, intemode and tentril had similar PHYE expression levels. Root had little PHYE expression. PHYE was found to have significantly lower expression levels in the youngest sample versus the oldest within a tissue type. Additional studies will be needed to determine shade avoidance response.
Library of Congress Subject Headings
Grapes -- Genetics
Phytochrome
Format
application/pdf
Number of Pages
54
Publisher
South Dakota State University
Recommended Citation
Anderson, Drew Louis, "Vitis Riparia Phytochrome E Expression Differs with Tissue Type and Age" (2009). Electronic Theses and Dissertations. 1496.
https://openprairie.sdstate.edu/etd2/1496
