Genetic Study of Aureobasidium pullulans Using Mutagenesis and Random Amplified Polymorphic DNA Analysis

Author

Yong Zhang

Document Type

Thesis - University Access Only

Award Date

1994

Degree Name

Master of Science (MS)

Department / School

Biology and Microbiology

First Advisor

Carl A. Westby

Abstract

The objectives of this study were to: 1) Conduct mutagenesis using EMS and γ-irradiation to generate pullulan deficient (pul^-) mutants from wild type A. pullulans ATCC 42023 (pul⁺). 2) Use Random Amplified Polymorphic DNA (RAPD) analysis to detect polymorphisms between A. pullulans ATCC 42023 and puI^- mutants in order to generate possible probes for cloning pullulan biosynthesis related genes.

Library of Congress Subject Headings

Polysaccharides -- Synthesis
Cloning
Mutagenesis
DNA -- Analysis

Format

application/pdf

Publisher

South Dakota State University

Recommended Citation

Zhang, Yong, "Genetic Study of Aureobasidium pullulans Using Mutagenesis and Random Amplified Polymorphic DNA Analysis" (1994). Electronic Theses and Dissertations. 20.
https://openprairie.sdstate.edu/etd2/20