Document Type
Dissertation - University Access Only
Award Date
2004
Degree Name
Doctor of Philosophy (PhD)
Department / School
Biology and Microbiology
First Advisor
Thomas P. West
Abstract
The goal of this project was the lysine augmentation of corn distillers' grains with solubles using a microbiological treatment process. Initially, it was necessary to isolate lysine-overproducing mutant strains of Escherichia coli K12 EMG2 and Saccharomyces cerevisiae ATCC 10274 and 26109. Using chemical mutagenesis and resistance to lysine analogs, it was possible to isolate mutant strains. Five mutants from E. coli and S.cerevisiae were isolated, their lysine overproduction was compared to their respective parent strains and statistically significant differences were observed. When these mutant strains were grown in thin stillage and compared to their respective parent strain, only yeast mutants 2a 12 and 1 b29 and the E. coli mutant strain EMS 1-40 exhibited statistically significant lysine overproduction. After the lysine overproducers were grown in filtered thin stillage and mixed with wet distillers' grains, the dried grains were assayed for lysine content. The E. coli mutant strain failed to increase the lysine content of the grains, while the yeast mutant strain 2a12 increased the lysine content of the grains. In conclusion, a benchtop scale treatment process using the yeast lysine overproducers could be used to improve the lysine content of dry distillers' grains with solubles and make the grains a more complete animal feed.
Library of Congress Subject Headings
Distillers feeds -- Microbiology.
Lysine in animal nutrition.
Corn as feed.
Publisher
South Dakota State University
Recommended Citation
Meyer, Rachel J., "Lysine Augmentation of Corn Distillers' Grains with Solubles Using a Microbiological Treatment Process" (2004). Electronic Theses and Dissertations. 1085.
https://openprairie.sdstate.edu/etd2/1085
Rights Statement
